What cloning sites will you use to clone the synthesized gene into pUC57? September 13, 2019 15:58 Updated We usually use blunt end ligation into EcoRV, but can also use any site you desired. Related articles We have the primers, do you need us to provide them to you or you will design and synthesize them? I received my gene in pUC57, why did you add extra base pair at both end of my sequence? How is a gene synthesized? Is it possible to share some helpful tips on handling the plasmid? What is the process of developing GenWandTM dsDNA?