If the recognition site is preceded by GA and followed by TC, or constitutes a CCWGG sequence (methylation site), you may not be able to digest the plasmid. Hence, it is recommended to avoid enzymes such as: XbaI (TCTAGA), ClaI (ATCGAT), ApaI (GGGCCC), AvaII (GGWCC), SfiI (GGCCNNNNNGGCC), StuI (AGGCCT). However, if you are unable to avoid using these enzymes on the methylated cloning sites for your plasmid, you may use a dam deficient strain such as GM2163 (E4105S), ER2925 or JM110 to amplify the DNA before digestion. Please inform us while placing the order.
Articles in this section
- Why do I see extra bands in my PCR product?
- Which vector do you use when synthesizing the gene?
- Which software can I use to open the sequence files of the synthesized gene?
- What QC data do I get for gene synthesis service?
- What options does GenScript offer for gene deliverables?
- What kind of bacterial strain is used in gene synthesis service and how should it be handled?
- What is the storage condition of genes/ constructs you made?
- What is the format you deliver the gene?
- What is the biggest gene you can synthesize?
- What is the biggest construct Genscript can handle?