I received my gene in pUC57, why did you add extra base pair at both end of my sequence? August 14, 2019 12:39 They are protective bases for blunt end ligation. Related articles How to store gene deliverables, including lyophilized plasmid, bacstab and glycerol stock? How should I amplify plasmid DNA from a bacstab or glycerol stock received from GenScript? I only have the vector map but not the sequence. Can you still process my request? What's the difference between ExpressPlus™ and SurePAGE™ gels? How is a gene synthesized?