I received my gene in pUC57, why did you add extra base pair at both end of my sequence? August 14, 2019 12:39 They are protective bases for blunt end ligation. Related articles How do I know if my plasmid is a high- or low copy number type? I want to try to do an experiment with your protein, but the bioassay you use for determining activity is not the same as my application. Will my application work with your protein? Does Catalog No. A00160 (Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb) cross react with rabbit? How should I amplify plasmid DNA from a bacstab or glycerol stock received from GenScript? Why am I seeing multiple or unexpected bands on a Western with my affinity purified antibody?