Calculate extinction coefficient of an oligo by either summing up the extinction coefficients of the individual bases times their number of occurrences. Alternatively, you can use a formula that takes into account the nearest neighbor effects. The algorithm for this calculation can be found on the web. http://biotools.nubic.northwestern.edu/OligoCalc.html Simply enter the sequence and the program will calculate the concentration of a 1 ml solution with A260 of 1.
Articles in this section
- Why should oligonucleotides be purified?
- Why does MALDI analysis of my oligos containing one or more Fluorescein-dTs give an incorrect mass even though they give only a single, fluorescent band on a PAGE gel?
- What shall I do with the RNA synthesis product from your company?
- What exactly is an OD?
- Sometimes I notice that the dried oligos have a brown color after drying. Is this the natural color of DNA or is the brown color a sign of contamination?
- I used oligos for cloning. When I sequenced a clone and found a mutation within the oligo sequence. Can mutations happen in synthetic oligos?
- How to store the synthetic Oligos?
- How should I resuspend my fluorescent dye-labeled oligos?
- How long will my fluorescent dye-labeled oligos last?
- How do you calculate the extinction coefficient of an oligo?