Yes. Fluorescent dye-labeled oligos are more prone to degradation than unmodified oligos. Their ability to fluoresce will decrease over time. Spin the tube at 1000 rpm for 5 minutes to ensure the oligos are at the bottom of the tube before opening. To ensure high quality, store the oligo dry at -20°C in small aliquots. Note that fluorescent dye-labeled oligos should be stored in the dark as light can slowly degrade the fluorescent moieties. For optimal long-term storage, it is recommended that the labeled oligonucleotides be stored dry at -20°C in the dark. If multiple experiments are planned using the same oligonucleotide, prepare aliquots, lyophilize all aliquots, and store the aliquots at -20°C. Use clean and sterile lab wares for all handlings.
Articles in this section
- Why should oligonucleotides be purified?
- Why does MALDI analysis of my oligos containing one or more Fluorescein-dTs give an incorrect mass even though they give only a single, fluorescent band on a PAGE gel?
- What shall I do with the RNA synthesis product from your company?
- What exactly is an OD?
- Sometimes I notice that the dried oligos have a brown color after drying. Is this the natural color of DNA or is the brown color a sign of contamination?
- I used oligos for cloning. When I sequenced a clone and found a mutation within the oligo sequence. Can mutations happen in synthetic oligos?
- How to store the synthetic Oligos?
- How should I resuspend my fluorescent dye-labeled oligos?
- How long will my fluorescent dye-labeled oligos last?
- How do you calculate the extinction coefficient of an oligo?