Our default vector for gene synthesis is pUC57. The difference between pUC57 and pUC57 simple vector is that pUC57 simple doesn't have the MCS. As the map showed, it only has NdeI and EcoRV enzyme sites. Normally, we will clone the insert into the vector at the middle of EcoRV site. If you choose NdeI/HindIII as the cloning site, you can use pUC57. The NdeI and HindIII in pUC57 are unique.
Articles in this section
- Why can't I cut this plasmid with XbaI and I can successfully cut other constructs with XbaI?
- Why customers get incorrect colonies when the CloneEZ reaction is transformed?
- Why customers are not able to get sufficient colonies when the CloneEZ reaction is transformed?
- Why do you add some extra bases outside of cloning sites? Do these bases interfere with any downstream applications?
- What's the method Genscript uses for subcloning?
- What is your definition of “subcloning” , “PCR cloning” and “customized cloning”?
- What is your cloning/subcloning method in generating constructs for customer?
- What is the main difference between cloneEZ and Invitrogen Gateway cloning?
- I need a common expression vector. Do you have any in stock?
- What E.coli competent do you usually use for cloning?