Our default vector for gene synthesis is pUC57. The difference between pUC57 and pUC57 simple vector is that pUC57 simple doesn't have the MCS. pUC57 simple vector has two commonly used sites: NdeI and EcoRV enzyme sites. Normally, for this vector, the gene will be cloned to EcoRV. If you choose NdeI/HindIII as the cloning site, you can use pUC57. The NdeI and HindIII on pUC57 are unique. Alternatively, Nde-HindIII sites can be synthesized additionally at both ends of the gene, and then insert it into the pUC57 simple vector.
Articles in this section
- Why do I see extra bands in my PCR product?
- Which vector do you use when synthesizing the gene?
- Which software can I use to open the sequence files of the synthesized gene?
- What QC data do I get for gene synthesis service?
- What options does GenScript offer for gene deliverables?
- What kind of bacterial strain is used in gene synthesis service and how should it be handled?
- What is the storage condition of genes/ constructs you made?
- What is the format you deliver the gene?
- What is the biggest gene you can synthesize?
- What is the biggest construct Genscript can handle?