How do you make site-directed or permutation scanning library? August 14, 2019 12:00 We synthesize the gene and then amplify with degenerate PCR primers. Related articles How do you make randomized and degenerate libraries? How to calculate the diversity of a site-directed or permutation scanning library? Which protocol do you use for mutagenesis, is it quick change? How do you confirm the library size? The variety of the library? What product will you deliver to me when the mutagenesis project completed?