How do you make site-directed or permutation scanning library? August 14, 2019 12:00 We synthesize the gene and then amplify with degenerate PCR primers. Related articles How do you make randomized and degenerate libraries? What QC is done after plasmid prep? How many continuous "R" can we synthesize in a peptide? What is the main difference between cloneEZ and Invitrogen Gateway cloning? How to calculate the diversity of a site-directed or permutation scanning library?