How do you make randomized and degenerate libraries? September 13, 2019 07:40 Updated We use error prone PCR. Related articles How do you make site-directed or permutation scanning library? What QC is done after plasmid prep? Which protocol do you use for mutagenesis, is it quick change? How do you determine/confirm the library size or the variety of the library? Why target gene products are seen in control sample with no template?