How do you make randomized and degenerate libraries? September 13, 2019 07:40 Updated We use error prone PCR. Related articles How do you make site-directed or permutation scanning library? What QC is done after plasmid prep? What is CRISPR? How do you confirm the library size? The variety of the library? Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?