How do you make randomized and degenerate libraries? October 20, 2023 07:35 Updated We use error prone PCR to product random mutant library and degenerated primer (NNN/NNK) to make degenerated mutant library. Related articles How do you make site-directed or permutation scanning library? What QC is done after plasmid prep? Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability? What is the diversity of randomized and degenerate library? The product sequence doesn't completely match the expected sequence. How can this result be improved?