This is because the primer area also has the possibility of mismatch. There are two kinds of possibilities for this mismatch: synthetic error and primer area mismatch. To determine this, more than 2 clones can be selected for sequencing verification, and if the results are identical, they should be synthetic errors. If the base is different in the same position of the primer, it should be the primer area mismatch.
Articles in this section
- Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
- Why there is a difference between my sequencing data and the one from the references?
- Why the short PCR product is not suitable for direct sequencing?
- Why target gene products are seen in control sample with no template?
- Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
- Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
- Why is the sequence of primers in the sequencing results different from the expected sequence?
- Why is my gene sequence different from the standard sequence?
- Why I got a sequencing result that was not I want?
- Why does the sequencing primer need to be site-specific on the DNA template?