After we receive the bacteria liquid or flat sample, we will expand the culture of the bacteria.
If it is not successful, it may be that the concentration of the bacteria is not high enough, the bacteria liquid is not fresh enough or the resistance is not correct, or the bacteria culture conditions are more special, such as Agrobacterium tumefaciens.
You can directly supply more than 4ml cultured bacteria solution, direct extract or provide plasmid samples.
In addition, if the copy number of the plasmid is low or the constructed fragment is larger, it is possible that the laboratory batch operation can not reach the effect that you can provide plasmid samples.
