After we receive the bacteria liquid or plate sample, we will expand the culture of the bacteria.
If it is not successful, it might due to the concentration of the bacteria is not enough, the bacteria liquid is not fresh enough or the resistance is incorrect, and the bacteria culture conditions are more special, such as Agrobacterium tumefaciens. we would direct conduct extraction if you provide more than 4ml bacteria culture, or we suggest you provide plasmids sample.
In addition, if the copy number of the plasmid is low or the constructed fragment is larger, it is possible that the laboratory batch operation can not as good as that you can provide plasmid samples.