We recommend customers to provide the bacteria and let us extract the plasmid to ensure purity and quantity of DNA samples. If the customer provides a DNA sample, we need to check the purity and quantity of samples. If the amount of DNA provided is insufficient, we need to amplify the plasmid. A conversion fee may be charged. The quality of DNA varies from different extraction methods. DNA purity and quantity should be checked when sequencing samples are PCR products. PCR products must be recovered by gel extraction, otherwise good sequencing results will not be obtained.
Articles in this section
- Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
- Why there is a difference between my sequencing data and the one from the references?
- Why the short PCR product is not suitable for direct sequencing?
- Why target gene products are seen in control sample with no template?
- Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
- Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
- Why is the sequence of primers in the sequencing results different from the expected sequence?
- Why is my gene sequence different from the standard sequence?
- Why I got a sequencing result that was not I want?
- Why does the sequencing primer need to be site-specific on the DNA template?