We recommend customers to provide the bacteria and we extract the plasmid to ensure the DNA sample is stable. It's also accpetable if DNA samples are provided by the customers. However, we need to check the purity and quantity of samples. If the amount of customer supplied DNA is insufficient, we need to amplify the plasmid. A conversion fee may be required. The quality of DNA varies from different extraction methods. DNA purity and quantity should be checked when sequencing samples are PCR products. PCR products must be re-extraction by gel cutting, otherwise they will not be able to gurantee the sequencing results.
Articles in this section
- Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
- Why there is a difference between my sequencing data and the one from the references?
- Why the short PCR product is not suitable for direct sequencing?
- Why target gene products are seen in control sample with no template?
- Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
- Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
- Why is the sequence of primers in the sequencing results different from the expected sequence?
- Why I got a sequencing result that was not I want?
- Why does the sequencing primer need to be site-specific on the DNA template?
- Why does my sequencing fail?