1. sample name: to distinguish the sample.
2. sample type: different types of samples have different processing procedures.
3. sample description (vector full name, resistance, target fragment size , restriction site)
A. resistance: our company provides A+/Kan+. If you need other antibiotics, they can be provided directly and please indicate the use concentration.
B.The full name of the vector and the restriction site can facilitate the selection of universal primers for suitable vectors.
C. fragment size:to determine the number of reactions required for testing and confirm the size of the target band needed for the recovery of PCR samples.
4. primer description (universal / self contained, primer name, primer concentration, whether verified)
5. sequencing requirements: how to make sure that sequencing orders are arranged. ? DONOT UNDERSTAND
6. remarks (hairpin, GC% high, methylation, etc.): according to the information of the sample, different protocols should be used for sequencing, avoiding adjustment and prolonging the experimental period.
