Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
Why there is a difference between my sequencing data and the one from the references?
Why the short PCR product is not suitable for direct sequencing?
Why target gene products are seen in control sample with no template?
Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
Why is the sequence of primers in the sequencing results different from the expected sequence?
Why I got a sequencing result that was not I want?
Why does the sequencing primer need to be site-specific on the DNA template?
Why does my sequencing fail?

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How do the walking primers return?

August 13, 2019 12:23

The total amount of RPC synthesis is 2nmol.
Dry powder: 2nmol needs to be diluted to 100uM concentration. 20ul is added to the water. If the concentration is diluted to 10uM, 200ul water should be added.
The walking primer concentration after sequencing is about 3.2pmol/ul.