The resolution of mass spectrometry instrument is above 0.1%, so if there is base-pair missing/mutation, it should show in the mass spectrometry test results. However, it cannot be ruled out that the impurity of the primers is too low to be detected because of the limitation of MS instrument. So it is possible that the sequencing results show impure primers. We recommend that customers pick 2-3 clones for sequencing and the possibility for good results will be much higher.
Articles in this section
- Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
- Why there is a difference between my sequencing data and the one from the references?
- Why the short PCR product is not suitable for direct sequencing?
- Why target gene products are seen in control sample with no template?
- Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
- Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
- Why is the sequence of primers in the sequencing results different from the expected sequence?
- Why I got a sequencing result that was not I want?
- Why does the sequencing primer need to be site-specific on the DNA template?
- Why does my sequencing fail?