Based on the error rate of our DNA product, it is estimated that sequencing approximately 2-3 colonies for fragments <1000 bp and 4-6 colonies for fragments 1000-1800 bp will yield a sequence-perfect clone with 99% confidence. In some cases, due to the nature of DNA sequences, additional colony screenings may be necessary. When using two or more gene fragments for assembly and cloning, we strongly recommend screening more colonies or first confirming the correct individual gene fragment before proceeding with the assembly.
Articles in this section
- How do I improve the chance of gene sequence to pass difficult filter and successfully synthesized?
- Can I put N and K mixed bases in my gene fragment order?
- What shall I do if I do not get correct colonies during my cloning reaction using GenTitan gene fragments?
- Should I PCR amplify my gene fragments for downstream application?
- How do I design my Gene Fragment for restriction cloning?
- How much of DNA is delivered?
- How are gene fragments delivered?
- What is the QC step for GenTitan Gene Fragment?
- What are the adapter sequences used on the Adapter-On GenTitan gene fragments? What methods can I use to clone the GenTitan gene fragments with adaptors?
- Do you purify the gene fragments?