We advise against PCR amplification of the gene fragments received due to the purification method employed, which involves the use of beads. This bead-based purification process has the potential to result in the carryover of smaller DNA fragments. It is important to note that these smaller fragments have a tendency to amplify more efficiently during PCR, often leading to the appearance of smears or additional smaller bands in the amplified products.
Articles in this section
- How do I improve the chance of gene sequence to pass difficult filter and successfully synthesized?
- Can I put N and K mixed bases in my gene fragment order?
- What shall I do if I do not get correct colonies during my cloning reaction using GenTitan gene fragments?
- Should I PCR amplify my gene fragments for downstream application?
- How do I design my Gene Fragment for restriction cloning?
- How much of DNA is delivered?
- How are gene fragments delivered?
- What is the QC step for GenTitan Gene Fragment?
- What are the adapter sequences used on the Adapter-On GenTitan gene fragments? What methods can I use to clone the GenTitan gene fragments with adaptors?
- Do you purify the gene fragments?