Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step.
Although TFA is important, residual TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. If needed, please kindly download GenScript’s Review to get more idea about the Impact of TFA in the below link:
In general, most custom peptides are delivered as lyophilized TFA salts and can contain as much as 10%-45% TFA. To improve the study outcome, TFA removal is essential before starting your experiment.